Determination of Equilibrium Constants using NMR Spectrscopy

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چکیده

In this laboratory exercise we will measure a chemical equilibrium constant using key proton signals in a Nuclear Magnetic Resonance (NMR) spectrum. Those doing the “traditional” P Chem experiments will examine a keto-enol tautomerism, while those doing the “biophysical” experiments will look at the cis-trans equilibrium for a peptide bond. In each case, the relative concentration of the equilibrium species can be determined from the NMR signal strength. The relative concentrations can then be leveraged to determine the desired equilibrium constant. Solvent effects on the equilibrium constant will be examined.

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تاریخ انتشار 2012